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Clinical evaluation reveals low neutrophil dye reduction in group A streptococcal pharyngitis, indicating the NBT test cannot distinguish streptococcal from nonstreptococcal disease.
During peak clinical symptoms, 17 patients with culture proven group A streptococcal pharyngitis had less than 9% NBT-positive neutrophils. The NBT test thus does not appear to be useful in differentiating streptococcal from nonstreptococcal pharyngitis.
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Shapera et al. (1973) studied this question.
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