Key result
Decreased circulating miR-20b-5p and miR-330-3p levels associate with AF recurrence 1 year post-ablation.
Why the study?
Peripheral blood miRNA expression might not reflect cardiac phenomena because most miRNAs are expressed in multiple organs, leaving cardiac-specific circulating miRNA biomarkers for AF unidentified.
Do circulating miR-20b-5p and miR-330-3p predict atrial remodeling progression and arrhythmia recurrence after catheter ablation in AF patients?
Observational (n=153)
No
Do circulating miR-20b-5p and miR-330-3p predict atrial remodeling progression and arrhythmia recurrence after catheter ablation in AF patients?
p-value: p=<0.05
Circulating miR-20b-5p and miR-330-3p are identified as novel cardiac-specific biomarkers for atrial remodeling and AF recurrence post-ablation.
miR-20b-5p and miR-330-3p levels may aid post-ablation recurrence prediction; hypothesis-generating and requires prospective validation before clinical use.
BACKGROUND: Circulating microRNAs (miRNAs, miR) have been considered as biomarkers reflecting the underlying pathophysiology in atrial fibrillation (AF). Nevertheless, miRNA expression in the peripheral blood samples might not reflect a cardiac phenomenon since most miRNAs are expressed in numerous organs. This study aimed to identify the cardiac-specific circulating miRNAs as biomarkers for AF. METHODS: Plasma samples were obtained from a luminal coronary sinus catheter (CS, cardiac-specific samples) and femoral venous sheath (FV, peripheral samples) in patients with AF and paroxysmal supraventricular tachycardia (control, CTL) undergoing catheter ablation. The circulating miRNA profiles were analyzed by small RNA sequencing. Differently expressed miRNAs between AF and CTL were identified in each sample of the CS and FV; miRNAs exhibiting similar expression patterns in the CS and FV samples were selected as candidates for cardiac-specific biomarkers. The selected miRNAs were related to the outcome of catheter ablation of AF. RESULTS: Small RNA sequencing detected 849 miRNAs. Among the top 30 most differently expressed miRNAs between AF and CTL, circulating hsa-miR-20b-5p, hsa-miR-330-3p, and hsa-miR-204-5p had a similar pattern in the CS and FV samples. Another set of peripheral blood samples was obtained from AF patients undergoing catheter ablation (n = 141). The expression of the miR-20b-5p and miR-330-3p, but not the miR-204-5p, negatively correlated with the echocardiographic left-atrial dimension and was decreased in patients with AF recurrence as compared to those without AF recurrence during a 1-year follow-up. CONCLUSION: Circulating miR-20b-5p and miR-330-3p can be cardiac-specific biomarkers for atrial remodeling progression and arrhythmia recurrence after catheter ablation in AF patients.
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Harada et al. (2023) conducted an observational in Atrial fibrillation (n=153). Catheter ablation vs. Patients without atrial fibrillation recurrence was evaluated on Atrial fibrillation recurrence at 1 year (p=<0.05). Circulating miR-20b-5p and miR-330-3p levels were significantly decreased in patients with atrial fibrillation recurrence compared to those without recurrence at 1 year after catheter ablation.
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