Mechanisms of tropomyosin 3 in the development of malignant tumors

Fig. 1. Schematic representation of the human TPM3 gene (located in chromosome 1). Colored boxes represented coding exons 1 through 9, white boxes represented untranslated 5′ and 3′ UTR sequences, black boxes are exons common to all the genes which share a high degree of homology and lines represented introns.The names of the different tm antibodies (in gray) are located below the corresponding exons.

Fig. 2. The TPM3 expression profile across all tumor samples and paired normal tissues from the GEPIA database. Adrenocortical carcinoma (ACC), Breast invasive carcinoma (BRCA), Cholangio carcinoma (CHOL), Lymphoid Neoplasm Difuse Large B-cell Lymphoma (DLBC), Glioblastoma multiforme (GBM), Kidney Chromophobe (KICH), Kidney renal papillary cell carcinoma (KIRP), Brain Lower Grade Glioma (LGG), Lung adenocarcinoma (LUAD), Ovarian serous cystadenocarcinoma (OV), Pheochromocytoma and Paraganglioma (PCPG), Rectum adenocarcinoma (READ), Skin Cutaneous Melanoma (SKCM), Testicular Germ Cell Tumors (TGCT), Thymoma (THYM), Uterine Carcinosarcoma (UCS).

Fig. 3. Protein-protein interaction (PPI) network analysis of 10 interacting proteins correlated with TPM3. TSPAN16 (tetraspanin 16), TFG (TRK-fused gene), NTRK1 (neurotrophic tyrosine kinase receptor type 1), TNNI3 (troponin I type 3), TNNT1 (troponin t type 1), TNNT2 (troponin t type 1), TNNC1 (troponin c type 1), ACTC1 (actin alpha 1, cardiac muscle), TPM1 (tropomyosin 1), ACTA1 (actin alpha 1,skeleton muscle).

Fig. 4. According to the GEPIA database, it was further found that the data of 15 tumor samples and paired normal tissues had significant differences in TPM3 expression (red rectangles: tumors, black rectangles: paraneoplastic controls). A. The TPM3 gene expression of tumor samples were lower than paired normal tissues in BLCA (bladder urothelial carcinoma), number tumor samples = 404, number paired normal tissues = 28 (num(T) = 404; num(N) = 28), P ≤ 0.05. B. The TPM3 gene expression of tumor samples were lower than paired normal tissues in BRCA, num(T) = 1085; num(N) = 291, P ≤ 0.05. C. The TPM3 gene expression of tumor samples were lower than paired normal tissues in CESC (cervical squamous cell carcinoma and endocervical adenocarcinoma), num(T) = 305; num(N) = 13, P ≤ 0.05. D. The TPM3 gene expression of tumor samples were higher than paired normal tissues in CHOL,num(T) = 36; num(N) = 9, P ≤ 0.05. E. The TPM3 gene expression of tumor samples were lower than paired normal tissues in ESCA (esophageal squamous cell carcinoma), num(T) = 182; num(N) = 288, P ≤ 0.05. F. The TPM3 gene expression of tumor samples were lower than paired normal tissues in COAD (colon adenocarcinoma), num(T) =…

Fig. 5. The effect of different expressions of TPM3 on overall survival in some patients with malignant tumors from GEPIA database (blue lines: low TPM3 TPM, red lines: high TPM3 TPM). Low TPM3 TPM had a better OS than high TPM3 TMP in ACC (adrenocortical carcinoma), n (high) = 38, n (low) = 38, P ≤ 0.05. A. Low TPM3 TPM had a better OS than high TPM3 TMP in MESO (mesothelioma), n (high) = 41, n (low) = 41, P ≤ 0.05. B. Low TPM3 TPM had a better OS than high TPM3 TMP in CESC, n (high) = 146, n (low) = 146, P ≤ 0.05. C. Low TPM3 TPM had a better OS than high TPM3 TMP in LIHC, n (high) = 182, n (low) = 182, P ≤ 0.05. D. High TPM3 had a better OS than low TPM3 TMP in COAD, n (high) = 135, n (low) = 135, P ≤ 0.05.