In chronically infarcted rat hearts, a 30% reduction in palmitate oxidation and downregulation of fatty acid transporters (e.g., FAT/CD36 by 36%) correlated with reduced ejection fraction.
In a rat model of chronic myocardial infarction, the downregulation of cardiac fatty acid transporters and reduced fatty acid oxidation correlate with the severity of left ventricular dysfunction.
OBJECTIVES: Cardiac fatty acid uptake occurs predominantly via sarcolemmal transporter proteins; fatty acid translocase (FAT/CD36), plasma membrane fatty acid binding protein (FABPpm) and fatty acid transporter proteins (FATP) 1 and 6. We hypothesised that levels of the fatty acid transporters would be reduced in the chronically infarcted rat heart, in parallel with reduced dependence on fatty acid utilisation. METHODS AND RESULTS: In vivo left ventricular ejection fractions, measured using echocardiography, were 36% lower in rats six months after coronary artery ligation than in sham-operated control rats. In isolated, perfused, infarcted hearts, 3H-palmitate oxidation was 30% lower, and correlated with in vivo ejection fractions. As myocardial lipid incorporation was also reduced by 25%, total palmitate utilisation was 29% lower in the infarcted rat heart. The protein levels of the cardiac fatty acid transporters were reduced in the infarcted rat heart; FAT/CD36 by 36%, FABPpm by 12%, FATP6 by 21% and FATP1 by 26%, and the cytosolic fatty acid binding protein (cFABP) was 47% lower than in sham-operated rat hearts. Fatty acid transporter levels correlated with both palmitate oxidation rates and cardiac ejection fractions. CONCLUSIONS: Reductions in fatty acid oxidation and lipid incorporation rates were accompanied by downregulation of the cardiac fatty acid transporters. The metabolic shift away from fatty acid utilisation was proportional to the degree of functional impairment in the chronically infarcted rat heart.
Heather et al. (Sat,) conducted a other in Chronically infarcted rat heart. Coronary artery ligation vs. Sham-operated control was evaluated on Palmitate oxidation rate and fatty acid transporter levels. In chronically infarcted rat hearts, a 30% reduction in palmitate oxidation and downregulation of fatty acid transporters (e.g., FAT/CD36 by 36%) correlated with reduced ejection fraction.