What question did this study set out to answer?

To determine whether a targeted mutation introduced into the nonselectable autosomal c-abl gene in embryonic stem cells can be transmitted through the germ line of chimeric mice.

November 10, 1989Science

Germ-Line Transmission of a c- abl Mutation Produced by Targeted Gene Disruption in ES Cells

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Authors

PSPamela L. SchwartzbergNational Institutes of Health SGStephen P. GoffAaron Diamond AIDS Research Center ERElizabeth J. RobertsonCouncil of Science Editors

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Implication

Experimental study demonstrates germ-line transmission of a targeted c-abl mutation in chimeric mice, indicating the feasibility of heritable modification of nonselectable autosomal genes.

Key Points

To determine whether a targeted mutation introduced into the nonselectable autosomal c-abl gene in embryonic stem cells can be transmitted through the germ line of chimeric mice.
Introduced a targeted substitution mutation into the endogenous c-abl locus of murine embryonic stem (ES) cells via homologous recombination using exogenous DNA.
Generated chimeric mice from the targeted ES cells and bred male chimeras to evaluate germ-line transmission to progeny.
Multiple male chimeric mice successfully transmitted the targeted c-abl mutation to their offspring.
Demonstrated the practical feasibility of achieving germ-line transmission of targeted mutations in nonselectable autosomal genes.

Cite This Study

Schwartzberg et al. (1989) studied this question.

synapsesocial.com/papers/6a11bf540765515418178c99 https://doi.org/10.1126/science.2554496

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Implication

Experimental study demonstrates germ-line transmission of a targeted c-abl mutation in chimeric mice, indicating the feasibility of heritable modification of nonselectable autosomal genes.

Germ-Line Transmission of a c- abl Mutation Produced by Targeted Gene Disruption in ES Cells

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Implication

Key Points

Cite This Study

Germ-Line Transmission of a c- abl Mutation Produced by Targeted Gene Disruption in ES Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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