Laboratory evaluation demonstrates sensitive cytochemical staining techniques for differentiating monocytes and granulocyte subtypes, indicating potential value for clinical diagnostic applications.
Cytochemical methods for chloroacetate esterase, nonspecific esterase, peroxidase, and metachromasia were used to identify monocytes and neutrophilic, basophilic, or eosinophilic granulocytes. These methods are very simple, sensitive, and easily reproducible. Their reaction products are highly chromogenic and insoluble in most organic solvents. Any of these methods may be used independently or in combination with another cytochemical method to demonstrate a particular type of cell as desired. The specific aims and possible areas for clinical application of these cytochemical methods are discussed.
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Yam et al. (1971) studied this question.
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