What question did this study set out to answer?

To evaluate a flow cytometric method using fluorescein isothiocyanate-labeled annexin V to detect phosphatidylserine exposure on B cells undergoing apoptosis.

September 1, 1994Blood

Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis

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Authors

GKGerrit KoopmanBiomedical Primate Research Centre CRCP ReutelingspergerAmsterdam UMC Location University of Amsterdam GKGA KuijtenAmsterdam UMC Location University of Amsterdam

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Implication

Experimental study demonstrates that annexin V detects externalized phosphatidylserine during early B-cell apoptosis, indicating surface exposure precedes membrane disruption.

Key Points

To evaluate a flow cytometric method using fluorescein isothiocyanate-labeled annexin V to detect phosphatidylserine exposure on B cells undergoing apoptosis.
Cultured Burkitt lymphoma cell lines and freshly isolated human germinal center B cells under apoptosis-inducing conditions.
Measured surface phosphatidylserine binding with fluorescein isothiocyanate-labeled annexin V and assessed cell membrane integrity using ethidium bromide via flow cytometry.
Annexin V strongly stained all apoptotic cells exhibiting chromatin condensation, whereas normal viable cells remained annexin V negative.
Internucleosomal DNA fragmentation occurred exclusively within the annexin V-positive cell populations.
Phosphatidylserine exposure coincided with chromatin condensation and preceded the membrane damage detected by ethidium bromide dye uptake.

Cite This Study

Koopman et al. (1994) studied this question.

synapsesocial.com/papers/6a11d054cd406dcc8edcdf57 https://doi.org/10.1182/blood.v84.5.1415.bloodjournal8451415

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Implication

Experimental study demonstrates that annexin V detects externalized phosphatidylserine during early B-cell apoptosis, indicating surface exposure precedes membrane disruption.

Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis

Discussion

Member takes

Implication

Key Points

Cite This Study

Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis

Discussion

Member takes

Implication

Key Points

Cite This Study

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