What question did this study set out to answer?

To determine the cellular mechanisms and conditions that mediate the adherence of neutrophils to adult canine cardiac myocytes.

May 1, 1990Journal of Clinical InvestigationOpen Access

Neutrophil adherence to isolated adult canine myocytes. Evidence for a CD18-dependent mechanism.

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Authors

MEMark L. EntmanHeart Failure & Transplant KYKeith A. YoukerHeart Failure & Transplant SSScott B. ShappellMonroe Carell Jr. Children's Hospital

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Implication

In vitro study demonstrates CD18-dependent neutrophil adherence to cytokine-stimulated adult canine myocytes, indicating a receptor-mediated pathway driving inflammatory cardiac injury.

Key Points

To determine the cellular mechanisms and conditions that mediate the adherence of neutrophils to adult canine cardiac myocytes.
Isolated adult canine cardiac myocytes were co-incubated with isolated canine neutrophils under resting conditions or after stimulation with cytokines (IL-1, TNF), PMA, or chemotactic agents (PAF, zymosan-activated serum).
Evaluated the effects of protein synthesis inhibitors and an anti-CD18 monoclonal antibody (R15.7) on myocyte-neutrophil adhesion and neutrophil hydrogen peroxide (H2O2) generation.
Intercellular adhesion increased significantly only when both myocytes (via IL-1, TNF, or PMA) and neutrophils were co-stimulated, and protein synthesis inhibition reduced IL-1 beta-induced adhesion by >80%.
High adhesion driven by IL-1 beta and PAF produced substantial H2O2, whereas low-adhesion conditions yielded <5% of this H2O2 production.
Treatment with an anti-CD18 monoclonal antibody (R15.7) inhibited stimulated neutrophil-myocyte adhesion by >95% and reduced H2O2 production by >90%.

Cite This Study

Entman et al. (1990) studied this question.

synapsesocial.com/papers/6a11e7ebdb195b84738e18b2 https://doi.org/10.1172/jci114596

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Implication

In vitro study demonstrates CD18-dependent neutrophil adherence to cytokine-stimulated adult canine myocytes, indicating a receptor-mediated pathway driving inflammatory cardiac injury.

Neutrophil adherence to isolated adult canine myocytes. Evidence for a CD18-dependent mechanism.

Discussion

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Implication

Key Points

Cite This Study

Neutrophil adherence to isolated adult canine myocytes. Evidence for a CD18-dependent mechanism.

Discussion

Member takes

Implication

Key Points

Cite This Study

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