July 20, 1984
View Full PaperThe Structure of Rat Preproatrial Natriuretic Factor as Defined by a Complementary DNA Clone
Structured PICO
P
Population
Rat preproatrial natriuretic factor (preproANF) complementary DNA clone
I
Intervention
Nucleotide sequence analysis
O
Outcome
Structure of rat preproANF
Determined the structure of rat preproANF, identifying its composition and the location of ANF at the carboxyl terminus.
Abstract
The structure of rat preproatrial natriuretic factor ( preproANF ) was determined by nucleotide sequence analysis of an ANF complementary DNA clone. PreproANF is composed of a hydrophobic leader segment (20 amino acids), a precursor containing one glycosylation site (106 amino acids), and ANF (24 amino acids). Atrial natriuretic factor is located at the carboxyl terminus of the precursor molecule. The human, mouse, and rat genomes each contain a single ANF gene which is highly conserved.