What question did this study set out to answer?

This study aimed to develop a scalable method for comprehensive DNA methylation profiling using a Tn5-based approach.

May 24, 2026Nature CommunicationsOpen Access

A scalable Tn5-based method for genome-wide DNA methylation profiling in development and disease

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Authors

HHHanrong HuUniversity of Geneva NSNahuel SimonetUniversity of Geneva EBEce Naz BilgicUniversity of Geneva

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Implication

Randomized trial demonstrates a Tn5-based method for DNA methylation profiling in development and disease, implying a cost-effective approach for epigenetic research.

Key Points

This study aimed to develop a scalable method for comprehensive DNA methylation profiling using a Tn5-based approach.
Developed C me CUT&Tag using Tn5-based approach for specific targeting of methylated DNA.
Enabled low-depth sequencing and optional cytosine conversion for base-pair resolution.
Characterized DNA methylation in human stem cells, organoids, zebrafish embryogenesis, and tumor biopsies.
C me CUT&Tag demonstrated strong concordance with bisulfite sequencing.
Enabled the classification of brain tumor samples into distinct methylation subtypes.
Facilitated genome-wide DNA methylation profiling with reduced input and sequencing requirements.

Cite This Study

Hu et al. (2026) studied this question.

synapsesocial.com/papers/6a12964948a0ea1665673035 https://doi.org/10.1038/s41467-026-73325-4

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Implication

Randomized trial demonstrates a Tn5-based method for DNA methylation profiling in development and disease, implying a cost-effective approach for epigenetic research.

A scalable Tn5-based method for genome-wide DNA methylation profiling in development and disease

Discussion

Member takes

Implication

Key Points

Cite This Study

A scalable Tn5-based method for genome-wide DNA methylation profiling in development and disease

Discussion

Member takes

Implication

Key Points

Cite This Study

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