Plasma Small Extracellular Vesicle Cardiac miRNA Expression in Patients with Ischemic Heart Failure, Randomized to Percutaneous Intramyocardial Treatment of Adipose Derived Stem Cells or Placebo: Subanalysis of the SCIENCE Study

Figures

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  Figure 1. Small EV concentration (A), vesicle size (B), spectrum of vesicle size (C), protein concentration (D), vesicle to protein ratio (E), RNA concentration (F), vesicle to RNA ratio (G), and expression of CD63, CD81, CD9 and albumin (H) in sEVs-EQ, sEVs-UC 1 mL, sEVs-UC 0.5 mL, sEVs-UC 0.25 mL, microvesicles (MVs) and plasma.

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  Figure 2. Small EV protein concentration in the overall patient cohort (A), small EV size in ADSC and placebo patients (B), and the overall patient cohort (C).

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  Figure 3. Correlation of small EV concentration/mL with troponin (A), EF (B), and ESV (C). Correlation of small EV to protein ratio with troponin (D), EF (E), ESV (F) and EDV (G). Correlation of small EV to RNA ratio with troponin (H), EF (I), ESV (J), EDV (K). Correlation of small EV protein to RNA ratio with NT-proBNP (L) and EQ-5D-5L (M).

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  Figure 4. Small EV miR-24 (A), miR-21 (B), and miR-126 (C), and correlations amongst themselves (D–F).

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  Figure 5. Correlation of NT-proBNP with miR-24 (A), miR-126 (B), and miR-21 (C). Correlation of troponin with miR-24 (D) and miR-21 (E). Correlation of LV mass with miR-24 (F) and miR-21 (G) and 6MWT distance with miR-21 (H).

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  Figure 6. Study flow.

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  Figure 7. Overview of plasma preparation for small EV isolation.