This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
No takes yet. Share an insight, caveat, or question.
Mary C. Phelan (2006) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: