Key result
Zinc finger nucleases and designer zinc finger proteins represent powerful tools for precise genomic manipulation and regulation of endogenous gene expression, with emerging therapeutic applications.
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Zinc finger nucleases and related fusion proteins serve as highly specific tools for genomic editing and transcriptional regulation, with broad applications in research and potential gene therapies.
Recent work has shown that it is possible to target regulatory elements to DNA sequences of an investigator's choosing, increasing the armamentarium for probing gene function. In this review, we discuss the development and use of designer zinc finger proteins (ZFPs) as sequence specific tools. While the main focus of this review is to discuss the attachment of the FokI nuclease to ZFPs and the ability of the resulting fusion protein (termed zinc finger nucleases (ZFNs)) to genomically manipulate a gene of interest, we will also cover the utility of other functional domains, such as transcriptional activators and repressors, and highlight how these are being used as discovery and therapeutic tools.
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Davis et al. (2010) reported a review. Zinc Finger Nucleases (ZFNs) was evaluated. Zinc finger nucleases and designer zinc finger proteins represent powerful tools for precise genomic manipulation and regulation of endogenous gene expression, with emerging therapeutic applications.
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