What question did this study set out to answer?

To develop a high-yield method using cationic colloidal silica for isolating intact plasma membrane sheets to facilitate transbilayer protein mapping.

August 1, 1983Journal of Biological ChemistryOpen Access

Coating cells with colloidal silica for high yield isolation of plasma membrane sheets and identification of transmembrane proteins.

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Authors

LCL K ChaneyUniversity of Massachusetts Amherst BJBruce S. JacobsonUniversity of Massachusetts Amherst

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Implication

In vitro study demonstrates high-yield plasma membrane isolation using colloidal silica in Dictyostelium discoideum, highlighting its utility for selective transmembrane protein mapping.

Key Points

To develop a high-yield method using cationic colloidal silica for isolating intact plasma membrane sheets to facilitate transbilayer protein mapping.
Coated intact Dictyostelium discoideum cells with cationic colloidal silica particles and a polyanion pellicle to stabilize the external membrane surface.
Lysed cells and isolated dense, open plasma membrane sheets via rapid centrifugation.
Assessed membrane purity using marker enzymes and identified transmembrane proteins using lactoperoxidase iodination and autoradiography.
Plasma membrane sheets were isolated with a 70% to 80% yield and a 10- to 17-fold purification alongside low cytoplasmic contamination.
The silica pellicle remained intact post-lysis, shielding up to 92% of the external membrane surface from chemical or enzymatic attack and allowing selective sided labeling.

Cite This Study

Chaney et al. (1983) studied this question.

synapsesocial.com/papers/6a12ec2a8f1bac20a09e8a61 https://doi.org/10.1016/s0021-9258(17)44606-0

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Implication

In vitro study demonstrates high-yield plasma membrane isolation using colloidal silica in Dictyostelium discoideum, highlighting its utility for selective transmembrane protein mapping.

Coating cells with colloidal silica for high yield isolation of plasma membrane sheets and identification of transmembrane proteins.

Discussion

Member takes

Implication

Key Points

Cite This Study

Coating cells with colloidal silica for high yield isolation of plasma membrane sheets and identification of transmembrane proteins.

Discussion

Member takes

Implication

Key Points

Cite This Study

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