What question did this study set out to answer?

To establish a reproducible and gentle trace iodination procedure for labeling proteins without compromising their immunological properties.

July 16, 2009International Archives of Allergy and Applied Immunology

A Method of Trace Iodination of Proteins for Immunologic Studies

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Authors

PMPatricia J. McConaheyScripps Health FDFrank J. DixonUniversity of North Carolina at Chapel Hill

Discussion

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Implication

Experimental study demonstrates a trace iodination method for proteins in immunological models, highlighting preserved antigenic reactivity for diagnostic assays.

Key Points

To establish a reproducible and gentle trace iodination procedure for labeling proteins without compromising their immunological properties.
Formulated a chemical radioiodination procedure designed for the trace incorporation of radioactive iodine into purified proteins.
Optimized reagent conditions to achieve effective isotope labeling while minimizing structural damage or protein denaturation.
Achieved efficient and reproducible incorporation of trace iodine isotopes into target protein molecules.
Maintained native antigenic integrity and specific antibody-binding capacity suitable for sensitive immunological investigations.

Cite This Study

McConahey et al. (2009) studied this question.

synapsesocial.com/papers/6a13084c8f1bac20a09ebdc3 https://doi.org/10.1159/000229699

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July 16, 2009International Archives of Allergy and Applied Immunology

A Method of Trace Iodination of Proteins for Immunologic Studies

View Full Paper

Ask AI

Bookmark

Authors

PMPatricia J. McConaheyScripps Health FDFrank J. DixonUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Experimental study demonstrates a trace iodination method for proteins in immunological models, highlighting preserved antigenic reactivity for diagnostic assays.

Key Points

To establish a reproducible and gentle trace iodination procedure for labeling proteins without compromising their immunological properties.
Formulated a chemical radioiodination procedure designed for the trace incorporation of radioactive iodine into purified proteins.
Optimized reagent conditions to achieve effective isotope labeling while minimizing structural damage or protein denaturation.
Achieved efficient and reproducible incorporation of trace iodine isotopes into target protein molecules.
Maintained native antigenic integrity and specific antibody-binding capacity suitable for sensitive immunological investigations.

Cite This Study

McConahey et al. (2009) studied this question.

synapsesocial.com/papers/6a13084c8f1bac20a09ebdc3 https://doi.org/10.1159/000229699

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