Replacement of 10% saturated fat with either linoleic acid or alpha-linolenic acid in high-fat diets increased cranial left ventricular wall thickness and myocyte cross-sectional area in rats, without altering myocardial function.
Does dietary fatty acid composition modify obesity-induced myocardial structure and function in fat-fed rats?
In a rat model of high-fat feeding, replacing a portion of saturated fat with polyunsaturated fatty acids (LA or ALA) is associated with early left ventricular wall thickening and myocyte hypertrophy without functional changes.
Absolute Event Rate: 0.23% vs 0.203%
p-value: p=0.021
BACKGROUND: Obesity increases the risk for development of cardiomyopathy in the absence of hypertension, diabetes or myocardial ischemia. Not all obese individuals, however, progress to heart failure. Indeed, obesity may provide protection from cardiovascular mortality in some populations. The fatty acid milieu, modulated by diet, may modify obesity-induced myocardial structure and function, lending partial explanation for the array of cardiomyopathic phenotype in obese individuals. METHODS: Adult male Sprague-Dawley rats were fed 1 of the following 4 diets for 32 weeks: control (CON); 50% saturated fat (SAT); 40% saturated fat + 10% linoleic acid (SAT+LA); 40% saturated fat + 10% α-linolenic acid (SAT+ALA). Serum leptin, insulin, glucose, free fatty acids and triglycerides were quantitated. In vivo cardiovascular outcomes included blood pressure, heart rate and echocardiographic measurements of structure and function. The rats were sacrificed and myocardium was processed for fatty acid analysis (TLC-GC), and evaluation of potential modifiers of myocardial structure including collagen (Masson's trichrome, hydroxyproline quantitation), lipid (Oil Red O, triglyceride quantitation) and myocyte cross sectional area. RESULTS: Rats fed SAT+LA and SAT+ALA diets had greater cranial LV wall thickness compared to rats fed CON and SAT diets, in the absence of hypertension or apparent insulin resistance. Treatment was not associated with changes in myocardial function. Myocardial collagen and triglycerides were similar among treatment groups; however, rats fed the high-fat diets, regardless of composition, demonstrated increased myocyte cross sectional area. CONCLUSIONS: Under conditions of high-fat feeding, replacement of 10% saturated fat with either LA or ALA is associated with thickening of the cranial LV wall, but without concomitant functional changes. Increased myocyte size appears to be a more likely contributor to early LV thickening in response to high-fat feeding. These findings suggest that myocyte hypertrophy may be an early change leading to gross LV hypertrophy in the hearts of "healthy" obese rats, in the absence of hypertension, diabetes and myocardial ischemia.
Jeckel et al. (2011) studied Dietary obesity (n=22). High saturated fat + PUFA diets (SAT+LA and SAT+ALA) vs. Control diet (CON) and 50% saturated fat diet (SAT) was evaluated on Cranial left ventricular wall thickness during diastole (LVWcr/d) (p=0.021). Replacement of 10% saturated fat with either linoleic acid or alpha-linolenic acid in high-fat diets increased cranial left ventricular wall thickness and myocyte cross-sectional area in rats, without altering myocardial function.