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Genes for the luciferase enzyme of Vibrio harveyi were isolated in Escherichia coli by a general method in which nonluminous, transposon insertion mutants were used. Conditions necessary for light production in E. coli were examined. Stimulation of transcription of the genes for luciferase (lux A and lux B) was required for efficient synethesis of luciferase. To enhance transcription bacteriophage promoter elements were coupled to the cloned lux gene fragments.
Belas et al. (1982) studied this question.