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The specific binding of anions to oxidized horse‐heart cytochrome c has been studied by two methods: measuring the effect of ion concentration on the redox potential, and directly by gel nitration. The anions investigated were: chloride, phosphate, ADP and sulfanilate. The binding constants estimated are in the range of 1–10 mM −1 , and the number of sites per protein molecule are 1 to 2. The specificity of anion binding to the oxidized protein is demonstrated.
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Margalit et al. (1973) studied this question.
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