November 20, 2020Scientific ReportsOpen Access

Acute pathophysiological myocardial changes following intra-cardiac electrical shocks using a proteomic approach in a sheep model

Q: What is the clinical evidence from this study?

Population: Healthy (n=10). Intervention: Intra-cardiac electrical shocks vs. No shock delivery (control). Primary outcome: Myocardial proteome modifications.

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Key result

Intra-cardiac shocks in sheep induce acute myocardial proteome changes linked to cellular damage and oxidative stress.

n=10

Why the study?

ICD shocks reduce mortality but are paradoxically associated with increased mortality, prompting investigation into immediate myocardial protein changes after shocks.

Does intra-cardiac electrical shock induce acute pathophysiological myocardial changes at the protein level in a healthy sheep model?

Population

10 healthy male sheep

Comparison

5 consecutive shocks at 41 J vs no shock delivery

Design

Animal experimental study

Authors

ABAlexandre BodinElectrophysiology VLValérie LabasUniversité de Tours ABArnaud BissonElectrophysiology

Discussion

Member takes

Implication

Does not yet inform ICD management; hypothesis-generating for shock-related myocardial injury in humans.

Structured PICO

Does intra-cardiac electrical shock induce acute pathophysiological myocardial changes at the protein level in a healthy sheep model?

Population

10 healthy male adult sheep (Ovis aries)

Intervention

Surgical implantation of an implantable cardioverter-defibrillator (ICD) with delivery of 5 consecutive biphasic maximal energy shocks at 41 J synchronized on the R-wave

Comparator

Surgical implantation of an ICD without any shock delivery

Outcome

Myocardial changes at the protein level immediately after ICD electrical shocks, measured by MALDI-TOF MS tissue profiling, top-down HR-MS, and bottom-up analysissurrogate

Intra-cardiac electrical shocks induce acute myocardial tissue injury and significant proteome modifications, providing a potential mechanistic explanation for the paradoxical deleterious effects of ICD shocks.

Limitations

Samples were collected within minutes following electrical shocks, meaning some cellular machinery mechanisms may not have been fully involved.
A healthy adult sheep model was used, whereas ICDs are mainly implanted in heart failure patients with diseased myocardium.
Functional changes at later time points were not evaluated to observe potential reversibility or involvement in chronic heart failure.

Cite This Study

Bodin et al. (2020) studied Healthy (n=10). Intra-cardiac electrical shocks vs. No shock delivery (control) was evaluated on Myocardial proteome modifications. Intra-cardiac electrical shocks in a sheep model induced significant acute myocardial proteome modifications associated with cellular damage, metabolic remodeling, and oxidative stress.

synapsesocial.com/papers/6a153e22cb0379474a820c2b https://doi.org/10.1038/s41598-020-77346-x

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Acute pathophysiological myocardial changes following intra-cardiac electrical shocks using a proteomic approach in a sheep model

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Acute pathophysiological myocardial changes following intra-cardiac electrical shocks using a proteomic approach in a sheep model

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider