Key result
Recombinant fibroblast Tm isoform 5b dramatically increased calcium sensitivity compared to Tm5a or skeletal Tm, shifting the activation mid-point by almost an order of magnitude.
Population
Recombinant fibroblast tropomyosin isoforms 5a and 5b, and rabbit skeletal muscle proteins
Comparison
Tropomyosin isoform 5b (Tm5b, containing exon 6a) vs Tropomyosin isoform 5a and skeletal tropomyosin
Design
Preclinical
Authors
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Cautious interpretation required for HCM; leaves open whether Tm exon 6 variants alter calcium handling in vivo.
Tropomyosin exon 6 sequence significantly affects the calcium regulation of troponin, providing a mechanistic basis for how familial hypertrophic cardiomyopathy mutations in this region alter calcium regulation.
Maytum et al. (2004) studied Familial hypertrophic cardiomyopathy. Recombinant fibroblast Tm isoforms 5a and 5b vs. Skeletal Tm was evaluated on Calcium sensitivity of acto-myosin regulation and troponin binding. Recombinant fibroblast Tm isoform 5b dramatically increased calcium sensitivity compared to Tm5a or skeletal Tm, shifting the activation mid-point by almost an order of magnitude.
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