Sequence preservation of osteocalcin protein and mitochondrial DNA in bison bones older than 55 ka

Research Article| December 01, 2002 Sequence preservation of osteocalcin protein and mitochondrial DNA in bison bones older than 55 ka Christina M. Nielsen-Marsh; Christina M. Nielsen-Marsh 1Fossil Fuels and Environmental Geochemistry, Newcastle Research Group, Drummond Building, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK Search for other works by this author on: GSW Google Scholar Peggy H. Ostrom; Peggy H. Ostrom 2Department of Geological Sciences, Michigan State University, East Lansing, Michigan 48824, USA Search for other works by this author on: GSW Google Scholar Hasand Gandhi; Hasand Gandhi 2Department of Geological Sciences, Michigan State University, East Lansing, Michigan 48824, USA Search for other works by this author on: GSW Google Scholar Beth Shapiro; Beth Shapiro 3Department of Zoology, University of Oxford, Oxford OX1 3PS, UK Search for other works by this author on: GSW Google Scholar Alan Cooper; Alan Cooper 3Department of Zoology, University of Oxford, Oxford OX1 3PS, UK Search for other works by this author on: GSW Google Scholar Peter V. Hauschka; Peter V. Hauschka 4Harvard Schools of Medicine and Dental Medicine, Children's Hospital, Boston, Massachusetts 02115, USA Search for other works by this author on: GSW Google Scholar Matthew J. Collins Matthew J. Collins 5Fossil Fuels and Environmental Geochemistry, Newcastle Research Group, Drummond Building, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK Search for other works by this author on: GSW Google Scholar Author and Article Information Christina M. Nielsen-Marsh 1Fossil Fuels and Environmental Geochemistry, Newcastle Research Group, Drummond Building, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK Peggy H. Ostrom 2Department of Geological Sciences, Michigan State University, East Lansing, Michigan 48824, USA Hasand Gandhi 2Department of Geological Sciences, Michigan State University, East Lansing, Michigan 48824, USA Beth Shapiro 3Department of Zoology, University of Oxford, Oxford OX1 3PS, UK Alan Cooper 3Department of Zoology, University of Oxford, Oxford OX1 3PS, UK Peter V. Hauschka 4Harvard Schools of Medicine and Dental Medicine, Children's Hospital, Boston, Massachusetts 02115, USA Matthew J. Collins 5Fossil Fuels and Environmental Geochemistry, Newcastle Research Group, Drummond Building, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK Publisher: Geological Society of America Received: 25 Apr 2002 Revision Received: 15 Aug 2002 Accepted: 16 Aug 2002 First Online: 02 Jun 2017 Online ISSN: 1943-2682 Print ISSN: 0091-7613 Geological Society of America Geology (2002) 30 (12): 1099–1102. https://doi.org/10.1130/0091-7613(2002)030<1099:SPOOPA>2.0.CO;2 Article history Received: 25 Apr 2002 Revision Received: 15 Aug 2002 Accepted: 16 Aug 2002 First Online: 02 Jun 2017 Cite View This Citation Add to Citation Manager Share Icon Share Facebook Twitter LinkedIn Email Permissions Search Site Citation Christina M. Nielsen-Marsh, Peggy H. Ostrom, Hasand Gandhi, Beth Shapiro, Alan Cooper, Peter V. Hauschka, Matthew J. Collins; Sequence preservation of osteocalcin protein and mitochondrial DNA in bison bones older than 55 ka. Geology 2002;; 30 (12): 1099–1102. doi: https://doi.org/10.1130/0091-7613(2002)030<1099:SPOOPA>2.0.CO;2 Download citation file: Ris (Zotero) Refmanager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentBy SocietyGeology Search Advanced Search Abstract We report the first complete sequences of the protein osteocalcin from small amounts (20 mg) of two bison bone (Bison priscus) dated to older than 55.6 ka and older than 58.9 ka. Osteocalcin was purified using new gravity columns (never exposed to protein) followed by microbore reversed-phase high-performance liquid chromatography. Sequencing of osteocalcin employed two methods of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS): peptide mass mapping (PMM) and post-source decay (PSD). The PMM shows that ancient and modern bison osteocalcin have the same mass to charge (m/z) distribution, indicating an identical protein sequence and absence of diagenetic products. This was confirmed by PSD of the m/z 2066 tryptic peptide (residues 1–19); the mass spectra from ancient and modern peptides were identical. The 129 mass unit difference in the molecular ion between cow (Bos taurus) and bison is caused by a single amino-acid substitution between the taxa (Trp in cow is replaced by Gly in bison at residue 5). Bison mitochondrial control region DNA sequences were obtained from the older than 55.6 ka fossil. These results suggest that DNA and protein sequences can be used to directly investigate molecular phylogenies over a considerable time period, the absolute limit of which is yet to be determined. You do not have access to this content, please speak to your institutional administrator if you feel you should have access.