Key result
Agonist-evoked stiffening of cultured airway smooth muscle cells requires both actin polymerization and myosin activation, neither of which is sufficient alone.
Population
Cultured airway smooth muscle (ASM) cells
Comparison
Serotonin stimulation with or without inhibitors… vs Baseline or absence of inhibitors
Design
Preclinical
Authors
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Cell culture findings require in vivo validation before clinical relevance; leaves open cytoskeletal mechanisms in intact airway disease.
Both actin polymerization and myosin activation are required for agonist-evoked stiffening of cultured airway smooth muscle cells.
An et al. (2002) studied Airway smooth muscle cell stiffening. Serotonin (5-HT) and various inhibitors vs. Absence of drugs / baseline was evaluated on Cell stiffness and F-actin formation. Agonist-evoked stiffening of cultured airway smooth muscle cells requires both actin polymerization and myosin activation, neither of which is sufficient alone.
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