Diblock copolymers enhance folding of a mechanosensitive membrane protein during cell-free expression

This is the authors' abstract. We don't add key points for this paper.

Significance Membrane protein folding is a critical step that underlies proper cellular function as well as the design of technologies like vesicle-based biosensors and artificial cells. Membrane composition is known to play a role in membrane protein folding; however, the specific mechanical properties of membranes that govern protein folding remain unclear. Using a highly elastic nonnatural amphiphile, we highlight the importance of a membrane mechanical property, membrane elasticity, on the spontaneous insertion and folding of a model α-helical membrane protein. Through this study, we gain a deeper understanding of cellular membrane protein folding and offer a potential approach to improve the production of membrane proteins through optimizing the mechanical properties of synthetic scaffolds present in cell-free reactions.