What question did this study set out to answer?

To directly determine the role of the factor VIII carboxyl-terminal C2 domain in mediating binding to phospholipids.

May 15, 1990BloodOpen Access

Synthetic factor VIII peptides with amino acid sequences contained within the C2 domain of factor VIII inhibit factor VIII binding to phosphatidylserine

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Authors

PFPA FosterScripps Health CFCA FulcherUniversity of Southern California RHR A HoughtenUniversity of Geneva

Discussion

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Implication

In vitro assay demonstrates that synthetic C2 domain peptides block factor VIII binding to phosphatidylserine, suggesting residues 2303–2332 form a critical phospholipid interaction site.

Key Points

To directly determine the role of the factor VIII carboxyl-terminal C2 domain in mediating binding to phospholipids.
Synthesized 26 overlapping peptides spanning the entire C2 domain of human factor VIII.
Assayed the ability of the synthetic peptides to inhibit the binding of purified human factor VIII to immobilized phosphatidylserine using an enzyme-linked immunosorbent assay (ELISA).
Three overlapping peptides (residues 2303–2317, 2305–2332, and 2308–2322) inhibited factor VIII binding to phosphatidylserine by greater than 90% at a concentration of 100 µmol/L.
A fourth partially overlapping peptide (residues 2318–2332) inhibited factor VIII binding to phosphatidylserine by 65% at 100 µmol/L.

Cite This Study

Foster et al. (1990) studied this question.

synapsesocial.com/papers/6a155b3b79ff98d0de4e7ff3 https://doi.org/10.1182/blood.v75.10.1999.1999

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Implication

In vitro assay demonstrates that synthetic C2 domain peptides block factor VIII binding to phosphatidylserine, suggesting residues 2303–2332 form a critical phospholipid interaction site.

Synthetic factor VIII peptides with amino acid sequences contained within the C2 domain of factor VIII inhibit factor VIII binding to phosphatidylserine

Discussion

Member takes

Implication

Key Points

Cite This Study

Synthetic factor VIII peptides with amino acid sequences contained within the C2 domain of factor VIII inhibit factor VIII binding to phosphatidylserine

Discussion

Member takes

Implication

Key Points

Cite This Study

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