In Vitro Properties of WMRK-gH625, a Novel Hybrid Peptide against Multidrug-Resistant Pathogens

group B reference and clinical strains, with variable MIC and MBC values depending on the strain. Time-kill assays confirmed concentration-dependent bactericidal effects, particularly at ≥ 2 × MIC. Interestingly, the peptide displayed minimal cytotoxicity, low hemolytic activity, and induced minimal oxidative stress in human cells, supporting its safety profile. These findings indicate that WMRK-gH625 synergistically enhances antibacterial efficacy through targeted membrane interaction and translocation, offering a promising strategy for combating AMR and developing next-generation peptide-based antimicrobials.