Key result
Angiotensin II stimulates steroidogenic and mitogenic effects in bovine adrenocortical zona fasciculata/reticularis cells, which are mediated by the AT1 receptor.
The steroidogenic and mitogenic effects of angiotensin II in bovine adrenocortical zona fasciculata/reticularis cells are mediated specifically by the AT1 receptor subtype.
Does not inform clinical adrenal management; extends AT1 mediation data to bovine zona fasciculata cells but leaves human translation open.
Primary cultures of bovine adrenocortical zona fasciculata/reticularis (zfr) cells responded to angiotensin II (AII) with a dose-dependent increase in [3H]thymidine incorporation into DNA. The effect was maximal at 100 nmol/liter AII, and was dose dependently inhibited by (sar1, ala8)-AII (saralasin) and DuP753, but not by PD123177. Both AII-stimulated cortisol secretion and phosphoinositidase C activity were also inhibited by saralasin and DuP753, but not by PD123177. Pharmacological analysis of the antagonism of AII-stimulated cortisol secretion by saralasin and DuP753 produced pA2 values of 8.79 and 7.02, respectively. Whereas the pA2 for saralasin agreed closely with previous measurements in other systems, DuP753 was at least one order of magnitude less potent in inhibiting the action of AII in bovine zfr cells compared to previous measurements in rabbit vascular smooth muscle. We conclude that the steroidogenic and mitogenic effects of AII in bovine zfr cells are mediated by the AT1 receptor.
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Clyne et al. (1993) studied this question. Angiotensin II vs. saralasin, DuP753, PD123177 was evaluated on [3H]thymidine incorporation into DNA, cortisol secretion, and phosphoinositidase C activity. Angiotensin II stimulates steroidogenic and mitogenic effects in bovine adrenocortical zona fasciculata/reticularis cells, which are mediated by the AT1 receptor.