Key result
Tiny percutaneous needle biopsy yielded significantly higher numbers of satellite cells from young volunteers (4,500 vs 2,183 cells/mg, p=0.04) compared to traditional needle aspiration biopsy.
Why the study?
Does tiny percutaneous needle biopsy improve the yield of satellite cells, RNA, and protein compared to needle aspiration biopsy in healthy volunteers?
RCT (n=34)
Open-label
Randomly selected
No
Does tiny percutaneous needle biopsy improve the yield of satellite cells, RNA, and protein compared to needle aspiration biopsy in healthy volunteers?
May enhance satellite cell yields for muscle research in young volunteers; leaves open broader applicability and RNA/protein benefits.
Needle biopsy is widely used to obtain specimens for physiological, anatomical and biochemical studies of skeletal muscle (SM). We optimized a procedure which we termed tiny percutaneous needle biopsy (TPNB), to efficiently gather good numbers of human satellite cells and single dissociated fibers for the functional study of skeletal muscle; these samples permit isolation of high-quality RNA and sufficient amounts of proteins to allow molecular analysis. Moreover, TPNB showed a clear advantage in that the technique was easier than other procedures used on healthy volunteers in human trials. TPNB is a very safe minor surgical procedure. It is less traumatic than needle aspiration biopsy, and significant complications are improbable. TPNB should become established as an important tool in the investigation of SM and may be employed to study various physiological aspects of SM in human subjects. We suggest that TPNB should also be used in the study of muscle diseases and disorders including muscular dystrophy, congenital myopathy, and metabolic defects.
No takes yet. Share an insight, caveat, or question.
Tiziana Pietrangelo (2010) conducted an RCT in Healthy volunteers (n=34). Tiny percutaneous needle biopsy (TPNB) vs. Needle aspiration biopsy (NAB) was evaluated on Satellite cell yield per mg of skeletal muscle in young volunteers (p=0.04). Tiny percutaneous needle biopsy yielded significantly higher numbers of satellite cells from young volunteers (4,500 vs 2,183 cells/mg, p=0.04) compared to traditional needle aspiration biopsy.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: