What does this research mean for the field?

In intact rat papillary muscle, the rapid inward sodium current exhibits a threshold potential of -55 mV, is blocked by tetrodotoxin with a half-maximal concentration of 6.3 µmol/l, and requires two time constants to accurately describe its inactivation kinetics. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The study aims to measure and analyze the kinetics of sodium current in intact rat papillary muscles.

December 1, 1988The Journal of PhysiologyOpen Access

Sodium current kinetics in intact rat papillary muscle: measurements with the loose‐patch‐clamp technique.

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Population

Intact rat papillary muscles and trabeculae excised from right or left ventricle

Design

Preclinical

Authors

HAH. AntoniPakuan University DBD BöckerUniversity of Freiburg RERoland EickhornUniversity of Freiburg

Discussion

Member takes

Implication

Extends Hodgkin-Huxley kinetics to intact rat ventricle; leaves open translation to human cardiac electrophysiology.

Key Points

The study aims to measure and analyze the kinetics of sodium current in intact rat papillary muscles.
Conducted experiments using the loose-patch-clamp technique on rat papillary muscles at 25 degrees C.
Recorded sodium currents, evaluating density and effects of tetrodotoxin.
Applied the Hodgkin-Huxley model to analyze kinetic data, estimating time constants for activation and inactivation.
Sodium current density was recorded at a mean of 5.9 +/- 0.5 mA/cm2.
Tetrodotoxin (TTX) produced half-maximal blockade of INa at 6.3 +/- 0.8 mumol/l.
Time constants for activation reached a maximum of about 0.4 ms near -55 mV.

Structured PICO

Population

Intact rat papillary muscles and trabeculae excised from right or left ventricle

Intervention

Loose-patch-clamp technique and tetrodotoxin (TTX) application

Outcome

Rapid inward sodium current (INa) kinetics, including activation and inactivation time constants and TTX dose-dependent blockadesurrogate

This preclinical study details the activation and inactivation kinetics of the rapid inward sodium current in intact rat ventricular muscle.

Cite This Study

Antoni et al. (1988) studied this question.

synapsesocial.com/papers/6a15922f37103a43379fed63 https://doi.org/10.1113/jphysiol.1988.sp017376

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Sodium current kinetics in intact rat papillary muscle: measurements with the loose‐patch‐clamp technique.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Sodium current kinetics in intact rat papillary muscle: measurements with the loose‐patch‐clamp technique.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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