What question did this study set out to answer?

To identify how alpha-SNAP and NSF interact with individual components of the neuronal SNARE fusion complex to drive its disassembly.

July 1, 1995Journal of Biological ChemistryOpen Access

The N-Ethylmaleimide-sensitive Fusion Protein and α-SNAP Induce a Conformational Change in Syntaxin

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Authors

PHPhyllis I. HansonThe Ohio State University HOHenning OttoTechnische Universität Ilmenau NBN R BartonHoward Hughes Medical Institute

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Implication

In vitro study reveals that NSF and alpha-SNAP induce an ATP-dependent conformational change in syntaxin, suggesting a primary mechanism for SNARE complex disassembly.

Key Points

To identify how alpha-SNAP and NSF interact with individual components of the neuronal SNARE fusion complex to drive its disassembly.
Assayed direct binding of alpha-SNAP and NSF to isolated recombinant SNARE components, including syntaxin 1A, SNAP-25, and synaptobrevin II.
Tested the effects of NSF and ATP on full-length syntaxin and a carboxyl-terminal syntaxin fragment containing residues 194–288.
alpha-SNAP bound directly to syntaxin 1A and weakly to SNAP-25, but displayed no binding to synaptobrevin II.
In the presence of ATP, NSF catalyzed a conformational change in syntaxin that disrupted binding to both alpha-SNAP and synaptobrevin.
NSF failed to alter binding to the carboxyl-terminal syntaxin fragment, demonstrating that the conformational rearrangement requires cooperation between syntaxin amino- and carboxyl-terminal domains.

Cite This Study

Hanson et al. (1995) studied this question.

synapsesocial.com/papers/6a1598e0814bf8ec9a4ed2d3 https://doi.org/10.1074/jbc.270.28.16955

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Implication

In vitro study reveals that NSF and alpha-SNAP induce an ATP-dependent conformational change in syntaxin, suggesting a primary mechanism for SNARE complex disassembly.

The N-Ethylmaleimide-sensitive Fusion Protein and α-SNAP Induce a Conformational Change in Syntaxin

Discussion

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Implication

Key Points

Cite This Study

The N-Ethylmaleimide-sensitive Fusion Protein and α-SNAP Induce a Conformational Change in Syntaxin

Discussion

Member takes

Implication

Key Points

Cite This Study

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