Reduced Growth and Inflammation in Lrp5−/− Mice Adipose Tissue

Figures

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  FIGURE 1. Mice body weight and mice adipose tissue weight. (A) Wt and Lrp5 −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. *p < 0.05, ***p < 0.001.

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  FIGURE 2. Low‐density lipoprotein receptors gene expression in mice and humans. (A) Lrp5 gene expression in adipose tissue of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis for (B) Lrp5 levels in VAT vs. VAT weight in Wt mice and (C) Lrp5 expression in SCAT vs. SCAT weights in Wt mice. (D) Lrp1 gene expression in adipose tissue of NC/HC Wt and Lrp5 −/− mice. Regression analysis for (E) Lrp1 levels in VAT vs. VAT weights in Wt and Lrp5 −/− mice and (F) Lrp1 levels in SCAT vs. SCAT weights in Wt and Lrp5 −/− mice. n = 8–12 mice/group. (G) Lrp5 and (H) Lrp1 gene expression in adipose tissue of lean and obese patients. n = 3–5 subjects/group. *p < 0.05, **p < 0.01, ***p < 0.001.

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  FIGURE 3. Adipocyte markers expression in mice. (A) Fabp4, (B) Lpl, and (C) Cd36 expression levels in adipose tissues of NC/HC Wt and Lrp5 −/− mice. Regression analysis of (D) Fabp4, (E) Lpl, and (F) Cd36 gene expression in VAT and SCAT vs. VAT and SCAT weights from Wt and Lrp5 −/− mice. n = 8–12 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001.

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  FIGURE 4. Macrophage markers in mice. (A) Cd68, (B) Cd11b, and (C) F4/80 genetic levels in adipose tissues of NC/HC Wt and Lrp5 −/− mice. Regression analysis of (D) Cd68, (E) Cd11b, and (F) F4/80 levels in VAT and SCAT vs. VAT and SCAT weights from Wt and Lrp5 − / − mice. n = 8–12 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001.

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  FIGURE 5. Pro‐inflammatory markers in mice adipose tissues. (A) Cd80, (C) Nos2, (E) Pcsk9, and (G) Ccr7 gene expression in adipose tissues of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis of (B) Cd80, (D) Nos2, (F) Pcsk9, and (H) Ccr7 gene expression in VAT and SCAT vs. VAT and SCAT weights in Wt and Lrp5 − / − mice. n = 8–12 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001.

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  FIGURE 6. Anti‐inflammatory markers in mice adipose tissues. (A) Cd163, (B) Cd206, and (C) Arg1 gene levels in adipose tissues of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis of (D) Cd163, (E) Cd206, and (F) Arg1 levels in VAT and SCAT vs. VAT and SCAT weights in Wt and Lrp5 −/− mice. n = 8–12 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001.