What question did this study set out to answer?

To determine whether taxol regulates gene expression in murine macrophages and mimics lipopolysaccharide-mediated activation pathways.

October 1, 1992The Journal of ImmunologyOpen Access

Taxol increases steady-state levels of lipopolysaccharide-inducible genes and protein-tyrosine phosphorylation in murine macrophages

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Authors

CMCarl L. MantheyJanssen (United States)MBMary E. BrandesUniversity of Iowa PPPin‐Yu PereraUniversity of Maryland, Baltimore

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Implication

In vitro study demonstrates taxol mimics lipopolysaccharide gene induction and tyrosine phosphorylation in murine macrophages, suggesting a shared inflammatory signaling pathway.

Key Points

To determine whether taxol regulates gene expression in murine macrophages and mimics lipopolysaccharide-mediated activation pathways.
Assessed steady-state mRNA expression of TNF-alpha, IL-1 beta, IP-10, D3, D7, and D8 in C3H/OuJ and LPS-hyporesponsive C3H/HeJ macrophages treated with taxol (1–30 µM) using Northern blot analysis.
Evaluated microtubule stabilization using immunofluorescence microscopy and analyzed rapid protein-tyrosine phosphorylation following taxol exposure.
Taxol (1–30 µM) induced bioactive TNF-alpha secretion and elevated mRNA levels across all six target genes in C3H/OuJ macrophages with kinetics and magnitude mirroring Escherichia coli K235 LPS.
LPS-hyporesponsive C3H/HeJ macrophages showed no detectable gene induction in response to taxol, despite displaying intact taxol-mediated microtubule stabilization.
Taxol rapidly triggered tyrosine phosphorylation of 41- and 42-kDa proteins, demonstrating an early intracellular signaling event shared directly with LPS.

Cite This Study

Manthey et al. (1992) studied this question.

synapsesocial.com/papers/6a15dde3caf7e3ea0ee3cccc https://doi.org/10.4049/jimmunol.149.7.2459

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Implication

In vitro study demonstrates taxol mimics lipopolysaccharide gene induction and tyrosine phosphorylation in murine macrophages, suggesting a shared inflammatory signaling pathway.

Taxol increases steady-state levels of lipopolysaccharide-inducible genes and protein-tyrosine phosphorylation in murine macrophages

Discussion

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Implication

Key Points

Cite This Study

Taxol increases steady-state levels of lipopolysaccharide-inducible genes and protein-tyrosine phosphorylation in murine macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

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