What question did this study set out to answer?

Determine the quantitative relationship between calcium ion concentration and bioluminescent light emission from the photoprotein aequorin across a wide concentration range.

March 11, 1977Science

Aequorin Luminescence: Relation of Light Emission to Calcium Concentration—A Calcium-Independent Component

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Authors

DADavid G. AllenUniversity of North Carolina at Chapel Hill JBJohn R. BlinksUniversity of Georgia FPFranklyn G. PrendergastMayo Clinic in Arizona

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Implication

Laboratory study demonstrates calcium-independent luminescence in purified aequorin at low ion concentrations, indicating a three-site binding mechanism.

Key Points

Determine the quantitative relationship between calcium ion concentration and bioluminescent light emission from the photoprotein aequorin across a wide concentration range.
Measured light emission from calcium-sensitive aequorin across calcium ion concentrations ranging from 10(-9) to 10(-2) molar.
Plotted log-log relationships of light emission against calcium concentration and evaluated fit using a two-state conformational model.
Light emission was entirely independent of calcium ion concentration at very low Ca2+ levels.
The log-log plot of light emission as a function of calcium ion concentration exhibited a maximum slope of approximately 2.5.
The full response curve was accurately characterized by a two-state transition model involving three calcium-binding sites.

Cite This Study

Allen et al. (1977) studied this question.

synapsesocial.com/papers/6a15fc60a215942ca9e3f4e8 https://doi.org/10.1126/science.841325

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Implication

Laboratory study demonstrates calcium-independent luminescence in purified aequorin at low ion concentrations, indicating a three-site binding mechanism.

Aequorin Luminescence: Relation of Light Emission to Calcium Concentration—A Calcium-Independent Component

Discussion

Member takes

Implication

Key Points

Cite This Study

Aequorin Luminescence: Relation of Light Emission to Calcium Concentration—A Calcium-Independent Component

Discussion

Member takes

Implication

Key Points

Cite This Study

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