What question did this study set out to answer?

To characterize the morphological and biochemical alterations that occur during the differentiation of unstimulated monocyte-like cells into mature macrophages.

January 1, 1965The Journal of Experimental MedicineOpen Access

The Differentiation of Mononuclear Phagocytes

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Authors

ZCZanvil A. CohnRockefeller Foundation BBBelinda BensonRockefeller Foundation

Discussion

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Implication

Laboratory study demonstrates lysosomal organelle accumulation and hydrolase induction during monocyte differentiation in mouse peritoneal cells, indicating organelle biogenesis drives maturation.

Key Points

To characterize the morphological and biochemical alterations that occur during the differentiation of unstimulated monocyte-like cells into mature macrophages.
Cultured homogeneous populations of monocyte-like cells isolated from the unstimulated mouse peritoneal cavity in vitro.
Assessed cytological modifications, organelle accumulation, and changes in the activity and content of acid phosphatase, cathepsin, and beta-glucuronidase.
Compared in vitro cellular differentiation profiles with mouse peritoneal phagocytes stimulated in vivo using bacterial lipopolysaccharide.
Cultured cells exhibited progressive increases in cell size without significant cell division, accompanied by the accumulation of mitochondria, lipid droplets, and acid phosphatase-reactive granules.
Total content and specific activity of acid phosphatase, cathepsin, and beta-glucuronidase increased markedly, with acid phosphatase showing more rapid and extensive accumulation than the other hydrolases.
In vivo stimulation with bacterial lipopolysaccharide elicited a comparable pattern of morphological maturation and lysosomal enzyme elevation.

Cite This Study

Cohn et al. (1965) studied this question.

synapsesocial.com/papers/6a16113edca1af9bedbf8e2a https://doi.org/10.1084/jem.121.1.153

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January 1, 1965The Journal of Experimental MedicineOpen Access

The Differentiation of Mononuclear Phagocytes

View Full Paper

Ask AI

Bookmark

Authors

ZCZanvil A. CohnRockefeller Foundation BBBelinda BensonRockefeller Foundation

Discussion

Member takes

Implication

Laboratory study demonstrates lysosomal organelle accumulation and hydrolase induction during monocyte differentiation in mouse peritoneal cells, indicating organelle biogenesis drives maturation.

Key Points

To characterize the morphological and biochemical alterations that occur during the differentiation of unstimulated monocyte-like cells into mature macrophages.
Cultured homogeneous populations of monocyte-like cells isolated from the unstimulated mouse peritoneal cavity in vitro.
Assessed cytological modifications, organelle accumulation, and changes in the activity and content of acid phosphatase, cathepsin, and beta-glucuronidase.
Compared in vitro cellular differentiation profiles with mouse peritoneal phagocytes stimulated in vivo using bacterial lipopolysaccharide.
Cultured cells exhibited progressive increases in cell size without significant cell division, accompanied by the accumulation of mitochondria, lipid droplets, and acid phosphatase-reactive granules.
Total content and specific activity of acid phosphatase, cathepsin, and beta-glucuronidase increased markedly, with acid phosphatase showing more rapid and extensive accumulation than the other hydrolases.
In vivo stimulation with bacterial lipopolysaccharide elicited a comparable pattern of morphological maturation and lysosomal enzyme elevation.

Cite This Study

Cohn et al. (1965) studied this question.

synapsesocial.com/papers/6a16113edca1af9bedbf8e2a https://doi.org/10.1084/jem.121.1.153

Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

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