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In order to deduce the unique structure of ox insulin it is necessary to know its molecular weight. In previous papers a value of 12 000 was assumedc since physical measurements suggested that this was the weight of the smallest unit that existed in solution, Recently, however, Harfenist Kupke Sanger Sanger Sanger, Thompson & Kitai, 1955) and is shown in 'core' was about 65 mg. It was further purified by dissolving Vol. 6o 543Q
Ryle et al. (Mon,) studied this question.
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