What does this research mean for the field?

Adenosine has no direct effect on basal contractility or action potentials in isolated mammalian ventricular myocytes, but it effectively antagonizes the stimulatory and arrhythmogenic effects of isoproterenol at physiologically relevant concentrations. Novelty: ClaimNovelty.CONFIRMATORY. Consensus alignment: ConsensusAlignment.SUPPORTS_CONSENSUS.

What question did this study set out to answer?

This research aims to examine how adenosine and isoproterenol affect the electrical and contractile properties of isolated ventricular myocytes.

September 1, 1983Circulation Research

Actions of adenosine and isoproterenol on isolated mammalian ventricular myocytes.

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Why the study?

Does adenosine modulate the electrophysiological and contractile effects of isoproterenol in isolated mammalian ventricular myocytes?

Key Points

This research aims to examine how adenosine and isoproterenol affect the electrical and contractile properties of isolated ventricular myocytes.
Enzymatically dispersed ventricular myocytes from bovine and guinea pig hearts were studied.
Action potentials and contractility were measured in response to adenosine and isoproterenol.
The influence of adenosine on the effects of isoproterenol was specifically assessed.
Isoproterenol significantly prolonged action potentials by 34-41% and increased sarcomere shortening by 3-fold.
Adenosine at 20-50 microM reduced the effect of isoproterenol, limiting the action potential increase to 8-9%.
Adenosine abolished sustained rhythmic activity usually triggered by isoproterenol-induced afterdepolarizations.

Structured PICO

Does adenosine modulate the electrophysiological and contractile effects of isoproterenol in isolated mammalian ventricular myocytes?

Population

Enzymatically dispersed ventricular myocytes from bovine and guinea pig hearts

Intervention

Adenosine (up to 0.2 mM) and isoproterenol (1-10 nM)

Comparator

Baseline/control (cells without adenosine/isoproterenol)

Outcome

Action potential parameters and basal contractility (sarcomere shortening)surrogate

Adenosine effectively antagonizes the stimulatory electrophysiological and contractile actions of isoproterenol in isolated ventricular myocytes, suggesting a modulatory role during ischemia or increased demand.

Discussion

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Implication

Adenosine blunts isoproterenol effects in myocytes; leaves open its modulatory role during ischemia in vivo.

Cite This Study

Belardinelli et al. (1983) studied this question.

synapsesocial.com/papers/6a16678e028422572a623dff https://doi.org/10.1161/01.res.53.3.287

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Actions of adenosine and isoproterenol on isolated mammalian ventricular myocytes.

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Actions of adenosine and isoproterenol on isolated mammalian ventricular myocytes.

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider