Research on the Mechanism of Action of Chlorin-based Photosensitizers in Photodynamic Therapy for Tumors

In recent years, researchers have improved the solubility, tumor enrichment ability and photodynamic effect of cyanine-based photosensitizers by modifying their skeletons, adding hydrophilic groups, targeting ligands or constructing nano-aggregates. However, there is less research on the mechanism of action of cyanine-based photosensitizers, and the development of cyanine-based photosensitizer derivatives has broad prospects. This thesis takes the mechanism of action of cyanine-based photosensitizers as the main line, not only designs a series of new derivatives through chemical synthesis and structural analysis, but also reveals their structure-activity relationship and biological characteristics through the determination of photophysical properties and reactive oxygen species generation experiments (MTT method).This work is conducive to clarifying the molecular action pathways of cyanine-based photosensitizers in PDT, and provides a theoretical basis and technical route for the development of efficient, low-toxic and transferable third-generation photosensitizers. Through the study of photosensitivity, the cell activity of the products is analyzed by mass spectrometry, and the molecular structure is determined by mass spectrometry and other methods. This is of great significance for promoting the research of high-end anti-cancer drugs in China and the development of the precision medical industry.