In situ functionalization of biotinylated microtubules with biotinylated antibodies via a streptavidin bridge enabled the detection of selective antigen binding on a moving molecular shuttle.
Demonstrates a general strategy for selective cargo binding on kinesin-powered molecular shuttles for biosensing applications.
A new shuttle mission: The design of molecular shuttles, which integrate the motor protein kinesin to propel microtubules (the associated polymeric filament), requires a general strategy to achieve selective cargo binding. We describe the in situ functionalization of biotinylated microtubules with biotinylated antibodies via a streptavidin bridge and demonstrate that selective binding of antigens can be detected via a double-antibody sandwich assay on the moving shuttle.
Ramachandran et al. (Tue,) reported a other. Molecular shuttles with kinesin and microtubules was evaluated on Detection of selective binding of antigens via a double-antibody sandwich assay. In situ functionalization of biotinylated microtubules with biotinylated antibodies via a streptavidin bridge enabled the detection of selective antigen binding on a moving molecular shuttle.