Key result
In situ functionalization of biotinylated microtubules with biotinylated antibodies via a streptavidin bridge enabled the detection of selective antigen binding on a moving molecular shuttle.
Demonstrates a general strategy for selective cargo binding on kinesin-powered molecular shuttles for biosensing applications.
This is the authors' abstract. We don't add key points for this paper.
A new shuttle mission: The design of molecular shuttles, which integrate the motor protein kinesin to propel microtubules (the associated polymeric filament), requires a general strategy to achieve selective cargo binding. We describe the in situ functionalization of biotinylated microtubules with biotinylated antibodies via a streptavidin bridge and demonstrate that selective binding of antigens can be detected via a double-antibody sandwich assay on the moving shuttle.
Answers are grounded in this paper and cite their sources.
Advances antigen detection on kinesin shuttles for biosensing; leaves open in vivo validation and clinical translation.
No takes yet. Share an insight, caveat, or question.
Ramachandran et al. (2006) studied this question. Molecular shuttles with kinesin and microtubules was evaluated on Detection of selective binding of antigens via a double-antibody sandwich assay. In situ functionalization of biotinylated microtubules with biotinylated antibodies via a streptavidin bridge enabled the detection of selective antigen binding on a moving molecular shuttle.
Synapse has enriched 10 related papers. Consider them for comparative context: