What question did this study set out to answer?

Determine whether helper and suppressor activities of T lymphocytes represent inherent properties of distinct, pre-programmed cellular subclasses distinguished by Ly surface antigens.

June 1, 1976The Journal of Experimental MedicineOpen Access

Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses.

View Full Paper

Ask AI

Bookmark

Authors

JJJohn J. JandinskiRutgers, The State University of New Jersey HCHarvey CantorBoston Biomedical Research Institute TTT TadakumaUniversity of Shizuoka

Discussion

Member takes

Implication

Experimental study reveals distinct helper and suppressor activities in isolated Ly1 and Ly23 T-cell subclasses, indicating these functions arise from separate differentiative pathways.

Key Points

Determine whether helper and suppressor activities of T lymphocytes represent inherent properties of distinct, pre-programmed cellular subclasses distinguished by Ly surface antigens.
Activated isolated resting T lymphocytes polyclonally in vitro using concanavalin A.
Separated and eliminated Ly1 or Ly23 subclasses before and after activation using subclass-specific Ly antisera.
Assayed in vitro helper and suppressor functions using sheep erythrocyte antibody responses and cell-mediated cytotoxicity against H-2 target cells.
Activated Ly23 cells suppressed antibody responses to sheep erythrocytes, secreted a soluble suppressive factor, and blocked cell-mediated cytotoxicity against H-2 target cells.
Activated Ly1 cells exclusively mediated helper function in anti-sheep erythrocyte antibody responses, which remained masked in unseparated populations by Ly23 suppressor activity.
Resting Ly1 and Ly23 cells isolated prior to mitogen stimulation exhibited the same respective helper and suppressor functions after activation, demonstrating antigen-independent lineage programming.

Cite This Study

Jandinski et al. (1976) studied this question.

synapsesocial.com/papers/6a17114ec7240d1a707bfa65 https://doi.org/10.1084/jem.143.6.1382

View Full Paper

Ask AI

Bookmark

Implication

Experimental study reveals distinct helper and suppressor activities in isolated Ly1 and Ly23 T-cell subclasses, indicating these functions arise from separate differentiative pathways.

Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses.

Discussion

Member takes

Implication

Key Points

Cite This Study

Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses.

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider