SSEA-1 isolates human endometrial basal glandular epithelial cells: phenotypic and functional characterization and implications in the pathogenesis of endometriosis

Anthony Valentijn; K. Palial; Hannan Al‐Lamee; Nicola Tempest; J Drury; Thomas von Zglinicki; Gabriele Saretzki; Patricia Murray; Caroline E. Gargett; D Hapangama

doi:10.1093/humrep/det285

July 11, 2013Human Reproduction

SSEA-1 isolates human endometrial basal glandular epithelial cells: phenotypic and functional characterization and implications in the pathogenesis of endometriosis

View at Publisher

Ask AI

Bookmark

Share

Key Points

To determine whether specific molecular and phenotypic markers can identify and isolate the regenerative basal epithelial compartment of the human endometrium.
Prospective observational analysis of full-thickness endometrium from hysterectomy samples of 115 premenopausal women, 15 post-menopausal women, and ectopic lesions from 20 women with endometriosis.
Immunohistochemistry was conducted for SSEA-1, SOX9, and β-catenin, and primary epithelial cells sorted into SSEA-1+/- subsets were assayed for telomerase activity, telomere length, pluripotency markers, and 3D spheroid formation.
Endometrial basal glandular epithelial cells selectively expressed nuclear SOX9, SSEA-1, and an activated subpopulation showed nuclear β-catenin.
Primary SSEA-1(+) epithelial cells exhibited significantly elevated telomerase activity, longer mean telomere lengths, reduced steroid receptor expression, and generated significantly more gland-like spheroids in 3D culture than SSEA-1(-) cells (P = 0.009).
Ectopic endometriotic lesions co-expressed SSEA-1 and nuclear SOX9, indicating shed basalis epithelial cells contribute to lesion development following retrograde menstruation.

No abstract yet

We only have this paper's title and publication details.

Read it at the source

Implication

Prospective observational study demonstrates SSEA-1 marks regenerative endometrial basalis cells in human tissue, indicating basalis cell dislocation drives endometriosis lesion formation.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Valentijn et al. (2013) studied this question.

synapsesocial.com/papers/6a1740733d715b2f195df007 https://doi.org/10.1093/humrep/det285

View at Publisher

Ask AI

Bookmark

Share

SSEA-1 isolates human endometrial basal glandular epithelial cells: phenotypic and functional characterization and implications in the pathogenesis of endometriosis

Key Points

No abstract yet

Implication

Discussion

Cite This Study

SSEA-1 isolates human endometrial basal glandular epithelial cells: phenotypic and functional characterization and implications in the pathogenesis of endometriosis

Key Points

No abstract yet

Implication

Discussion

Cite This Study

Keep reading

More related papers

More from Human Reproduction

Keep reading

More related papers

More from Human Reproduction