What question did this study set out to answer?

To develop and validate a liquid chromatography–tandem mass spectrometry method capable of simultaneously quantifying multiple forms of folate and folate catabolites in human serum.

April 10, 2009Clinical ChemistryOpen Access

Liquid Chromatography–Tandem Mass Spectrometry Analysis of Folate and Folate Catabolites in Human Serum

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To develop and validate a liquid chromatography–tandem mass spectrometry method capable of simultaneously quantifying multiple forms of folate and folate catabolites in human serum.
Obtained serum samples from 168 healthy blood donors and 39 patients with renal failure.
Pretreated serum with ascorbic acid, precipitated proteins using acetonitrile containing 13C-labeled internal standards, evaporated supernatant, and reconstituted analytes in water.
Quantified 5-methyltetrahydrofolate (5mTHF), 4-alpha-hydroxy-5-methyltetrahydrofolate (hmTHF), folic acid (FA), 5-formyltetrahydrofolate (5fTHF), p-aminobenzoylglutamate (pABG), and p-acetamidobenzoylglutamate (apABG) using LC-MS/MS.
Detection limits spanned 0.07 to 0.52 nmol/L with linearity up to 140 nmol/L for all analytes, correlating closely with microbiologic assay measurements (r² = 0.92) and pABG equivalent measurements (r² = 0.93).
Mean serum folate in 168 blood donors was 22.7 nmol/L (composed of 85.8% 5mTHF, 12.1% hmTHF, 2.1% FA, and 0.0% 5fTHF), with mean catabolite levels of 0.07 nmol/L for pABG and 0.47 nmol/L for apABG.
Serum concentrations of folate catabolites were 22- to 30-fold higher in 39 patients with renal failure than in healthy donors.

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Implication

Validation study demonstrates reliable quantification of folate species and catabolites in healthy donors and renal failure patients, highlighting marked catabolite accumulation in kidney disease.

Cite This Study

Hannisdal et al. (2009) studied this question.

synapsesocial.com/papers/6a17422651b167d07f5df220 https://doi.org/10.1373/clinchem.2008.114389

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Implication

Validation study demonstrates reliable quantification of folate species and catabolites in healthy donors and renal failure patients, highlighting marked catabolite accumulation in kidney disease.

Liquid Chromatography–Tandem Mass Spectrometry Analysis of Folate and Folate Catabolites in Human Serum

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Discussion

Implication

Cite This Study

Liquid Chromatography–Tandem Mass Spectrometry Analysis of Folate and Folate Catabolites in Human Serum

Key Points

Discussion

Implication

Cite This Study

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