Investigation of the kinetic growth parameters and biological activities of probiotic strain

Heyndrickxia coagulans M is a probiotic strain with antimicrobial activity against various foodborne pathogens and saprophytes, including bacteria and fungi. It has the potential for applications in probiotic preparations and plant protection. The strain was cultivated in three different nutrient media and the logistic curve and exponential mathematical models were employed to study the kinetics of the growth and sporulation processes. The strain demonstrated maximal value of the maximum specific growth rate of 0.122 h-1 in nutrient medium C and maximum specific sporulation rate of 0.215 h-1 in nutrient medium A. The obtained culture liquids were investigated for biological activities. Six ninhydrin-positive compounds with antifungal activity and different Rf-values were detected through thin-layer chromatography (Rf1 = 0.72; Rf2 = 0.81; Rf3 = 0.27; Rf4 = 0.96; Rf5 = 0.16 and Rf6 = 0.08).