Statistical analysis of low dose rate mouse experiments with WGS technology and quantitative reproduction of mutation frequency using the whack-a-mole model

We performed whole-genome sequencing (WGS) experiments using mice for several generations under the circumstances of radiation with low dose rates at Tokai University and the Institute for Environmental Sciences (IES) in Japan. The dose rates and total exposed periods were 0.05, 0.15, 1.0, and 20 mGy day −1 for 400, 400, 400, and 300 days, respectively. We performed a statistical analysis of the experimental results on the single-nucleotide variations (SNVs) for each mouse after four generations. The SNVs per generation were unchanged below the dose rate of 1 mGy day −1 within the 95% confidence level errors. The experimental results were reproduced by the Whack-a-Mole (WAM) model with the standard parameters obtained by the Russell mega-mouse experiments.