What question did this study set out to answer?

To determine the role of protein kinase C and its beta isoform in alpha-melanocyte stimulating hormone-induced tyrosinase expression and melanogenesis.

August 1, 1996Experimental Cell ResearchOpen Access

α-Melanocyte Stimulating Hormone-Induced Pigmentation Is Blocked by Depletion of Protein Kinase C

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Authors

HPHee‐Young ParkFuel Cells and Hydrogen VRVladimir RussakovskyBoston University YAYi AoBoston University

Discussion

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Implication

Experimental study reveals that protein kinase C depletion halts hormone-induced melanogenesis in mouse melanoma cells, indicating an essential regulatory role for the beta isoform.

Key Points

To determine the role of protein kinase C and its beta isoform in alpha-melanocyte stimulating hormone-induced tyrosinase expression and melanogenesis.
Treated S91 Cloudman mouse melanoma cells with 10^-7 M alpha-melanocyte stimulating hormone to track tyrosinase activity, mRNA, protein expression, and protein kinase C isoform levels over 6 days.
Depleted cellular protein kinase C using phorbol dibutyrate and compared responses in wild-type versus serially passaged hormone-unresponsive S91 cells.
Stimulation with alpha-MSH increased tyrosinase activity between Days 2 and 6, coinciding with increased tyrosinase mRNA, tyrosinase protein, and the PKC-beta isoform.
Treatment with phorbol dibutyrate eliminated 95% of PKC activity within 48 hours and completely blocked alpha-MSH-induced melanogenesis along with tyrosinase mRNA and protein upregulation.
Passaged S91 cells lacking responsiveness to alpha-MSH showed undetectable PKC-beta levels and failed to induce tyrosinase mRNA despite baseline tyrosinase protein levels matching responsive cells.

Cite This Study

Park et al. (1996) studied this question.

synapsesocial.com/papers/6a17eb52aeefdf6d9c13263f https://doi.org/10.1006/excr.1996.0251

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Implication

Experimental study reveals that protein kinase C depletion halts hormone-induced melanogenesis in mouse melanoma cells, indicating an essential regulatory role for the beta isoform.

α-Melanocyte Stimulating Hormone-Induced Pigmentation Is Blocked by Depletion of Protein Kinase C

Discussion

Member takes

Implication

Key Points

Cite This Study

α-Melanocyte Stimulating Hormone-Induced Pigmentation Is Blocked by Depletion of Protein Kinase C

Discussion

Member takes

Implication

Key Points

Cite This Study

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