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July 1, 1989AJP Renal Physiology

Inhibition of rat mesangial cell mitogenesis by nitric oxide-generating vasodilators

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Why the study?

Do NO-generating vasodilators inhibit mitogenesis in cultured rat mesangial cells?

Population

Cultured rat mesangial cells

Design

Preclinical

Authors

UGUttam GargChildren's Mercy HospitalAHAviv HassidUniversity of Tennessee Health Science Center

Discussion

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Implication

Inhibits rat mesangial mitogenesis in vitro; hypothesis-generating for NO/EDRF modulation of glomerular growth in vivo.

Key Points

  • This study aims to explore how nitric oxide-generating drugs affect DNA synthesis and cell proliferation in cultured mesangial cells.
  • Cultured rat mesangial cells treated with S-nitroso-N-acetylpenicillamine, sodium nitroprusside, and isosorbide dinitrate.
  • Assessment of serum-stimulated DNA synthesis and cell proliferation.
  • Measurement of cGMP levels and evaluation of reversibility and cell viability.
  • S-nitroso-N-acetylpenicillamine, sodium nitroprusside, and isosorbide dinitrate inhibited DNA synthesis and cell proliferation.
  • Treatment increased guanosine 3',5'-cyclic monophosphate (cGMP) levels dose-dependently.
  • Cell growth inhibition was reversible and not due to cytotoxicity.

Structured PICO

Do NO-generating vasodilators inhibit mitogenesis in cultured rat mesangial cells?

P
Population
Cultured rat mesangial cells
I
Intervention
Nitric oxide (NO)-generating drugs (S-nitroso-N-acetylpenicillamine, sodium nitroprusside, and isosorbide dinitrate)
O
Outcome
Serum-stimulated DNA synthesis and rate of cell proliferationsurrogate

NO-generating vasodilators inhibit rat mesangial cell mitogenesis in vitro, suggesting EDRF/NO may modulate mesangial cell growth in vivo.

Cite This Study

Garg et al. (1989) studied this question.

synapsesocial.com/papers/6a1820edaeefdf6d9c136401https://doi.org/10.1152/ajprenal.1989.257.1.f60
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells.1989 · 2,172 citations
  2. 2Antiproliferative effect of nitric oxide on rat glomerular mesangial cells via inhibition of mitogen‐activated protein kinase2001 · 17 citations
  3. 3Effects of endothelium-derived relaxing factor and nitric oxide on rat mesangial cells1990 · 124 citations
  4. 4Nitric oxide attenuates endothelin-1-induced activation of ERK1/2, PKB, and Pyk2 in vascular smooth muscle cells by a cGMP-dependent pathway2007 · 52 citations
  5. 5Inhibition of smooth muscle cell growth by nitric oxide and activation of cAMP-dependent protein kinase by cGMP1994 · 458 citations