Key result
A quantitative proteomics study evaluated data from 52 participants using various gel electrophoresis, HPLC, and mass spectrometry methods to determine the relative amounts of eight proteins.
This study evaluated and compared various gel electrophoresis, HPLC, and mass spectrometry methods used by 52 participants for determining relative protein abundance.
May inform proteomics method selection; leaves open optimal standardization for clinical translation.
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Calaghan et al. (2005) studied Proteomics methodology (n=52). Quantitative proteomics methods (gel electrophoresis, HPLC, mass spectrometry) was evaluated on Identity and relative amounts of eight proteins in two mixtures. A quantitative proteomics study evaluated data from 52 participants using various gel electrophoresis, HPLC, and mass spectrometry methods to determine the relative amounts of eight proteins.
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