Key result
Early cultivated mesenchymal cells from CVD patient cardiac fat show >90% stem cell marker expression.
Why the study?
The study was aimed to evaluate the immunophenotype of adipose tissue stem cells isolated from epicardial and perivascular fat depots in patients with coronary heart disease and acquired heart disease.
Observational
Mesenchymal cells isolated from epicardial and perivascular adipose tissue of patients with heart disease express surface markers characteristic of adipose tissue stem cells at early stages of cultivation.
Cardiac adipose-derived mesenchymal cells may offer a stem cell source; leaves open their regenerative utility in cardiovascular models.
The work was aimed to evaluate the immunophenotype of adipose tissue stem cells isolated from epicardial and perivascular fat depots in patients with coronary heart disease and acquired heart disease. In cell culture obtained from epicardial adipose tissue (EAT) and perivascular adipose tissue (PVAT) (second passage) of patients with both coronary heart disease and acquired heart disease, there was a high (over 90%) expression of membrane proteins characteristic of stem cells. In addition to a major cell population, the two minor were found both in EAT and in PVAT cell cultures: (1) CD90–, CD105+, CD34–/+, CD73+, CD45– (putative endothelial), and (2) CD90+, CD105–, CD34–, CD73–, CD45– (the smallest). Thus, at early stages of cultivation, mesenchymal cells of the stromal vascular fraction isolated from EAT and PVAT express surface markers characteristic of adipose tissue stem cells.
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Учасова et al. (2023) conducted an observational in Coronary heart disease and acquired heart disease. Cell culture from epicardial and perivascular adipose tissue was evaluated on Expression of membrane proteins characteristic of stem cells. Mesenchymal cells isolated from epicardial and perivascular adipose tissue of patients with cardiovascular diseases showed high (>90%) expression of stem cell surface markers at early cultivation.
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