Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae
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Key Points
To determine the subcellular localization and mechanistic function of the class E Vps protein Bro1/Vps31 within the multivesicular body sorting pathway in Saccharomyces cerevisiae.
Examined the subcellular distribution of the cytoplasmic protein Bro1/Vps31 in Saccharomyces cerevisiae.
Assessed endosomal association and dissociation of Bro1 across yeast strains harboring mutations in class E VPS genes, including VPS4 and ESCRT-III subunits.
Bro1 functions as a cytoplasmic factor that localizes to late endosomal membranes dependent specifically on the ESCRT-III subunit Snf7/Vps32.
Endosomal dissociation of Bro1 requires the AAA-ATPase Vps4, which similarly coordinates the release of the ESCRT-III complex.
Bro1 operates directly at endosomal membranes in concert with or downstream of the ESCRT-III complex during multivesicular body cargo sorting.
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Implication
Cell biology study reveals Bro1 associates with endosomal membranes via Snf7 in yeast, indicating coordinated action with ESCRT-III in sorting.