Key result
Thrombin cleaves cardiac troponin T between R68 and S69 during serum sample preparation, resulting in a 29-kDa fragment, whereas cTnT remains full-sized (35 kDa) in heparin plasma.
The 29-kDa fragment of cardiac troponin T commonly observed in AMI serum samples is an in vitro artifact caused by thrombin cleavage during serum preparation.
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Serum cTnT assays may detect in vitro artifacts; leaves open whether heparin plasma improves measurement accuracy in AMI.
Katrukha et al. (2017) studied Acute myocardial infarction. Serum sample preparation (thrombin exposure) vs. Heparin plasma or hirudin pretreatment was evaluated on cTnT degradation/cleavage. Thrombin cleaves cardiac troponin T between R68 and S69 during serum sample preparation, resulting in a 29-kDa fragment, whereas cTnT remains full-sized (35 kDa) in heparin plasma.
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