Plasma microrna expression profile for reduced ejection fraction in dilated cardiomyopathy

Figures

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  Figure 1. Flowchart of the study design strategy. This figure illustrates the experimental workflow of the study including screening, validation and EF status of DCM patients. Abbreviations: DCM, dilated cardiomyopathy; DCMMOD, dilated cardiomyopathy with ejection fraction ≥ 31–49%; DCMSEV, dilated cardiomyopathy with ejection fraction ≤ 30%; EF, ejection fraction.

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  Figure 2. Boxplots of miRNA expression levels, normalized to cel-miR-39-3p, in healthy subjects, idiopathic DCMMOD and DCMSEV. The analysis was carried out using qPCR. Data are presented in log2. Data represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations: DCMMOD, dilated cardiomyopathy with ejection fraction ≥ 31–49%; DCMSEV, dilated cardiomyopathy with ejection fraction ≤ 30%.

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  Figure 3. Correlation between the LVEF parameter and individual microRNAs for LVEF categorization in idiopathic DCM. (A-N) LVEF was independently negatively related with let-7a-5p (A), let 7 g-5p (B), miR-19b-3p (C), miR-25-3p (D), miR-29a-3p (E), miR-30b-5p (F), miR-30e-3p (G), miR-142-3p (H), miR-145-5p (I), miR-150-5p (J), miR-199a-3p (K), miR-210-3p (L), , miR-324-3p (M) and miR-660-5p (N) levels. Abbreviations: DCMMOD, dilated cardiomyopathy with ejection fraction ≥ 31–49%; DCMSEV, dilated cardiomyopathy with ejection fraction ≤ 30%.

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  Figure 4. ROC analysis for the multivariate logistic regression model predicting idiopathic DCMSEV patients. The mean AUC for the averaged ROC curves are presented. The data are presented as the AUC and 95% CI and corresponding sensitivity for a range of specificities. The AUC is 0.96 (95% CI: 0.884–1.00; p < 0.001). Abbreviations: AUC, area under the curve; CI, confidence interval; DCMSEV, dilated cardiomyopathy with ejection fraction ≤ 30%; ROC, receiver operating characteristic.

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  Figure 5. The miRNA-gene network for miRNAs associated with genes related to idiopathic DCMSEV. (A) Venn diagram showing overlap of gene targets of miR-130b-3p, miR-150-5p and miR-210-3p. (B) GO and KEGG analysis of miR-130b-3p, miR-150-5p and miR-210-3p target genes; the x-axis indicates KEGG pathway categories and the y-axis indicates the target numbers of genes. (C) miRNA-gene network for miR-210-3p. The network was built using 83 targeted genes and predicted interactions from the miRDB database. (D) PPI analysis performed using the STRING database for the target genes of miR-210-3p. The disconnected nodules are hidden. Bold lines indicate stronger association. Abbreviations: DCMSEV, dilated cardiomyopathy with ejection fraction ≤ 30%.