Key result
Pharmacological inhibition of Urotensin II with urantide promoted cardiac side population cell proliferation and improved cardiac dysfunction in mice during chronic pressure overload.
Why the study?
Does pharmacological inhibition of Urotensin II promote cardiac side population cell proliferation and improve cardiac dysfunction in mice with pressure overload?
Does pharmacological inhibition of Urotensin II promote cardiac side population cell proliferation and improve cardiac dysfunction in mice with pressure overload?
Pharmacological inhibition of urotensin II promotes the proliferation of intrinsic cardiac stem cells and improves cardiac function in a mouse model of pressure overload.
May support UII inhibition for pressure-overload repair in mice; leaves open translation to human HF therapy.
Objectives Cardiac side population cells (CSPs) are promising cell resource for the regeneration in diseased heart as intrinsic cardiac stem cells. However the relative low ratio of CSPs in the heart limited the ability of CSPs to repair heart and improve cardiac function effectively under pathophysiological condition. Which factors limiting the proliferation of CSPs in diseased heart are unclear. Here, we show that urotensin II (UII) regulates the proliferation of CSPs by c-Jun N-terminal kinase (JNK) and low density lipoprotein receptor-related protein 6 (LRP6) signalling during pressure overload. Methods Pressure overload was produced by the transverse aorta constriction (TAC). CSPs were isolated and purified by FACS analysis. ERK and JNK were examined by nanofluidic proteomic immunoassay (NIA) method. Results Pressure overload grealty upregulated UII level in plasma, UII receptor (UT) antagonist, urantide, promoted CSPs proliferation and improved cardiac dysfunction during chronic pressure overload. In cultured CSPs subjected to mechanical stretch (MS), UII significantly inhibited the proliferation by UT. Nanofluidic proteomic immunoassay (NIA) showed it is the JNK activation, but not the extracellular signal-regulated kinas (ERK) signalling, that involved in the UII-inhibited- proliferation of CSPs during pressure overload. Further analysis in vitro indicated UII-induced-phospho-JNK regulates phosphorylation of LRP6 in cultured CSPs after MS, which is important in the inhibitory effect of UII on the CSPs during pressure overload. Conclusions In couclusion, UII inhibited the proliferation of CSPs by JNK/LRP6 signalling during pressure overload. Pharmcological inhibition of UII promotes CSPs proliferation in mice, offering a possible therapeutic approach for cardiac failure induced by pressure overload.
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Chen et al. (2013) studied Cardiac failure induced by pressure overload. Urotensin II (UII) receptor antagonist (urantide) was evaluated on Proliferation of cardiac side population cells (CSPs) and cardiac dysfunction. Pharmacological inhibition of Urotensin II with urantide promoted cardiac side population cell proliferation and improved cardiac dysfunction in mice during chronic pressure overload.
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